Supplementary Fig. 1.

Phenotype of BMP4 knockout (BMP4 −/−) and control (BMP4 +/+) mice of different gender. (A) Growth curve of BMP4 knockout (BMP4 −/−) and control (BMP4 +/+) mice from 8 to 24 weeks. n = 8. (B) Comparison of BMP4 knockout and control mice of different gender. (C) Comparison of adipose tissue from BMP4 knockout and control mice of different gender. (D) Hematoxylin and eosin staining of liver from BMP4 knockout and control mice on HFD. Scale bar: 25 μm. (E) Fat index (percentage of fat pad weight to the whole body weight) of BAT in BMP4 knockout and control male (C) and female (D) mice. n = 8. Data were collected from mice on normal chow diet and expressed as means ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001.