Supplementary Fig. 3.

BMP4 regulation of ERα depends on levels of estrogens. (A) Western blotting analysis of ERα expression level in adipose tissue from male BMP4 knockout and control mice on HFD at age of 6 months. Relative grey intensity of the band was quantified using Image J software. n = 6. (B) Multipotent C3H10T1/2 cells stably expressing ERα-Flag were induced to differentiation and treated with 20 ng/ml BMP4 in presence of estradiol at different concentration for 12 h, and the protein level of ERα was examined with anti-Flag antibody by Western blot. Relative grey intensity of the band from 4 individual experiments was quantified using Image J software.