Fig. 2.
Kainic acid-induced neurodegeneration in CA3 region was reduced in Ryr1C3636A mice (a) KA (40 mg kg− 1, i.p.) triggered limbic seizures. n = 5–7. Dan, dantrolene. (b) Nissl-staining of hippocampus 24 h after KA injection. Scale bar: 500 μm. (c) Fluoro-Jade C staining of hippocampus. Dotted lines show the position of stratum pyramidale. Yellow (CA3) and white (CA1) boxed areas in upper panels are shown enlarged in bottom panels. n = 28–40 slices from 5 to 7 mice. Scale bar: 200 μm. Error bars indicate s.e.m. Data are analyzed for significance using ANOVA followed by a Tukey-Kramer post-hoc test. ** p < 0.01. (d) DAPI staining of hippocampus. n = 30–43 slices from 5 to 7 mice. Scale bar: 100 μm. Error bars indicate s.e.m. Data are analyzed for significance using ANOVA followed by a Tukey-Kramer post-hoc test. ** p < 0.01.