Fig. 3.

Involvement of NICR in NO-induced neuronal cell death (a) Effect of NO on neuron morphology (stained with anti-β-III tubulin antibody). Arrowheads indicate short and curly neurites. Scale bar: 100 μm. Graphs show the length of the longest neurite of each neuron; n = 52–116; error bars indicate s.e.m. Concentrations of NOC7 and dantrolene (Dan) were 500 μM and 10 μM, respectively. Data were analyzed for significance using ANOVA followed by a Tukey-Kramer post-hoc test. ** p < 0.01. (b) Cell viability examined by JC-1 assay. Scale bar: 10 μm. n = 49–65. Error bars indicate s.e.m. Data were analyzed for significance using ANOVA followed by a Tukey-Kramer post-hoc test. ** p < 0.01. See also Fig. S4.