Fig. 4.

Involvement of NICR in NO-induced mitochondrial fragmentation. Left panels show mitochondrial morphology before and 50 min after drug application. Glutamate (Glu; 100 μM) was used in positive control experiments. NOC7 concentration was 500 μM. Right upper panels show the representative time courses of fragmentation assessed by circularity of mitochondria. Right bottom panel show the average circularity of mitochondria between 55 and 60 min after drug application. Scale bar: 5 μm. n = 10–16. Data are analyzed for significance using t-test. *** p < 0.001.