Fig. 3.

H&E staining of adipose tissue and weights of non-adipose tissues in HFD-fed WT and tg/+ Tg-FABP4-RORα4 mice. (A) Representative images of WT and tg/+ SAT stained by H&E. (B) Quantification of cross sectional area of ~ 300 inguinal adipocytes from n = 4 littermate pairs of WT and tg/+ mice is shown as a histogram of the relative frequency of adipocyte sizes with bin intervals of 1000 μm² and mean and median area measurements. (C) Representative images of WT and tg/+ visceral epididymal adipose tissues stained by H&E. (D) Quantification of cross sectional area of ~ 300 epididymal adipocytes from n = 4 littermate pairs of WT and tg/+ mice is shown as a histogram of the relative frequency of adipocyte sizes with bin intervals of 2000 μm² and mean and median area measurements. Significance was calculated using unpaired two-tailed Student's t-test where *P < 0.05. (E) Representative image of liver dissected from chow-fed 32-week old WT and tg/+ mice. (F) DEXA scanning of dissected livers from chow-fed WT and tg/+ mice (n = 7 littermate pairs). (G) Mean relative mass/total weight ± S.E.M. of non-adipose tissues (liver, spleen and quadriceps muscle) dissected from chow-fed 32-week old WT and tg/+ littermate pairs (n = 7 littermate pairs). (H–I) Representative images of (H) liver and (I) spleen dissected from HFD-fed WT and tg/+ mice. (J) Mean relative mass/total weight ± S.E.M. of non-adipose tissues (liver, spleen and quadriceps muscle) dissected from WT and tg/+ littermate pairs after HFD (n = 9 littermate pairs). Statistical significance was calculated using unpaired two-tailed Student's t-test for where *P < 0.05; **P < 0.01.