Fig. 8.

FACS gating for SSC Somatostatin neurons: Data are from a representative sort; all runs produced similar patterns. Numbers represent percentages of cells within gates. A) All events are shown. DAPI(+) gate includes cells and excludes debris. B) NeuN-PE-Height/SideScatter-Height gate eliminates non-neurons and irregularly-shaped DAPI(+) events. C) Neurons are gated on Somatostatin-647-Height to select SOM(+) and SOM(−) neurons. D) 3 Control groups are gated: SOM(+), SOM(−)/Fos(+), and SOM(−)/Fos(−). E) 4 treated groups are gated: SOM(+)/Fos(+), SOM(+)/Fos(−), SOM(−)/Fos(+), SOM(−)/Fos(−). F) Histogram of cFos-immunoreactivity for all SOM(+) neurons from (R)-DOI (red) and control (blue) tissues.