Figure 2. Dynamic light conditions enhance pmf dependent phenotypes.

Whole plant fluorescent images were captured over three days under the illumination conditions displayed in Panel A and listed in Supplementary file 2. Plants were illuminated over the 16-hr photoperiod, shown as yellow filled areas representing the light intensity when present in A, under either a constant light intensity (day one), a sinusoidal photoperiod (day two), or a sinusoidal photoperiod interrupted by fluctuations in light intensity (day three). Square symbols in Panel A indicate each light intensity change. Steady-state fluorescence parameters were captured for each plant at the end of each light condition. Panels B,C and D represent the responses of LEF, q_E and q_I respectively (n ≥ 3). Data are shown as log₂-fold changes compared to the wild type. Kinetic data including wild type are shown in Figure 2—figure supplements 1–15. The rows were sorted in order of ascending g_H⁺ values measured as in Figure 1C. Panel E plots the dependence of the mean (n ≥ 3) of q_E against the linear electron flow (LEF) for each time point measured for the day. For visualization purposes the error bars have been omitted from E.

DOI: http://dx.doi.org/10.7554/eLife.16921.003

Figure 2—figure supplement 1. Whole plant fluorescence imaging phenotyping of minira 3–1 mutant.

Three week old plants were imaged over three consecutive 16-hr photoperiods and fluorescent measurements taken at the end of each light transition for LEF (A–C), q_E (D–F), and q_I (G–I) for Ws-2 and minira 3–1. Values represent mean of n ≥ 3 ± s.d., all of which were imaged in the same experiment. Timing and illumination are the same as Figure 2 and are detailed in Supplementary files 2a–c.

DOI: http://dx.doi.org/10.7554/eLife.16921.004

Figure 2—figure supplement 2. Increased pH-dependent quenching correlates with increased photoinhibitory quenching.

Whole plant fluorescent phenotypes were measured over three consecutive photoperiods. Day one consisted of a single irradiance level (open symbols), day two of sinusoidal irradiance (half filled symbols), and day three of sinusoidal irradiance interrupted by bright fluctuations (closed symbols). The pH-dependent fluorescence quenching (q_E) and photoinhibitory quenching (q_I) were integrated over time for each plant for each photoperiod to determine how prolonged exposure to an increased pmf influences the extent of photoinhibition. Data represent the integrated total for each day (n ≥ 3, ± s.d).

DOI: http://dx.doi.org/10.7554/eLife.16921.005

Figure 2—figure supplement 3. Whole plant fluorescence imaging phenotyping of minira 11–1 mutant.

For details, please refer to the legend of Figure 2—figure supplement 1.

DOI: http://dx.doi.org/10.7554/eLife.16921.006

Figure 2—figure supplement 4. Whole plant fluorescence imaging phenotyping of minira 14–1 mutant.

For details, please refer to the legend of Figure 2—figure supplement 1.

DOI: http://dx.doi.org/10.7554/eLife.16921.007

Figure 2—figure supplement 5. Whole plant fluorescence imaging phenotyping of minira 12–2 mutant.

For details, please refer to the legend of Figure 2—figure supplement 1.

DOI: http://dx.doi.org/10.7554/eLife.16921.008

Figure 2—figure supplement 6. Whole plant fluorescence imaging phenotyping of minira 8–1 mutant.

For details, please refer to the legend of Figure 2—figure supplement 1.

DOI: http://dx.doi.org/10.7554/eLife.16921.009

Figure 2—figure supplement 7. Whole plant fluorescence imaging phenotyping of minira 6–2 mutant.

For details, please refer to the legend of Figure 2—figure supplement 1.

DOI: http://dx.doi.org/10.7554/eLife.16921.010

Figure 2—figure supplement 8. Whole plant fluorescence imaging phenotyping of minira 4–2 mutant.

For details, please refer to the legend of Figure 2—figure supplement 1.

DOI: http://dx.doi.org/10.7554/eLife.16921.011

Figure 2—figure supplement 9. Whole plant fluorescence imaging phenotyping of minira 6–1 mutant.

For details, please refer to the legend of Figure 2—figure supplement 1.

DOI: http://dx.doi.org/10.7554/eLife.16921.012

Figure 2—figure supplement 10. Whole plant fluorescence imaging phenotyping of minira 7–1 mutant.

For details, please refer to the legend of Figure 2—figure supplement 1.

DOI: http://dx.doi.org/10.7554/eLife.16921.013

Figure 2—figure supplement 11. Whole plant fluorescence imaging phenotyping of minira 3–2 mutant.

For details, please refer to the legend of Figure 2—figure supplement 1.

DOI: http://dx.doi.org/10.7554/eLife.16921.014

Figure 2—figure supplement 12. Whole plant fluorescence imaging phenotyping of minira 4–1 mutant.

For details, please refer to the legend of Figure 2—figure supplement 1.

DOI: http://dx.doi.org/10.7554/eLife.16921.015

Figure 2—figure supplement 13. Whole plant fluorescence imaging phenotyping of minira 9–1 mutant.

For details, please refer to the legend of Figure 2—figure supplement 1.

DOI: http://dx.doi.org/10.7554/eLife.16921.016

Figure 2—figure supplement 14. Whole plant fluorescence imaging phenotyping of minira 4–3 mutant.

For details, please refer to the legend of Figure 2—figure supplement 1.

DOI: http://dx.doi.org/10.7554/eLife.16921.017

Figure 2—figure supplement 15. Whole plant fluorescence imaging phenotyping of minira 12–3 mutant.

For details, please refer to the legend of Figure 2—figure supplement 1.

DOI: http://dx.doi.org/10.7554/eLife.16921.018

Figure 2—figure supplement 16. Whole plant fluorescence imaging phenotyping of minira 2–2 mutant.

For details, please refer to the legend of Figure 2—figure supplement 1.

DOI: http://dx.doi.org/10.7554/eLife.16921.019