Table 2.

Table comparing Q140 mice and WT mice for the EM results on the total overall area occupied by ChAT+ dendritic profiles in dorsolateral striatum, the average area of individual ChAT+ dendritic profiles in striatum, and the number of ChAT+ dendritic profiles in striatum. Note that all three parameters show a reduction for cholinergic dendrites in Q140 mice. The Q140 and WT mice are also compared for VGLUT2+ synaptic terminals on ChAT+ dendritic profiles per unit area of striatum, and the abundance of VGLUT2+ terminals per unit area of ChAT+ dendrite. These results show that the frequency of VGLUT2+ terminals ending on ChAT+ dendrites per unit area of striatum is significantly reduced, but their abundance per unit area of ChAT+ dendrites is not significantly reduced. These results show that cholinergic neurons possess fewer and shorter dendritic branches in Q140 mice, and as a result individual cholinergic striatal neurons receive fewer synaptic terminals from thalamus.

EM Data on Cholinergic Neuron Dendrites and VGLUT2+ Terminals on Cholinergic Dendrites

Morphological Parameter – Cholinergic Dendrites	WT Mean (±SEM)	Q140 Mean (±SEM)	Q140 as Percent of WT	P Value*
Number of ChAT+ Dendritic Profiles (per μm2)	0.0417 ±0.002	0.0357 ±0.001	85.7%*	0.019
Average Size of Individual ChAT+ Dendrites (μm2)	1.874 ±0.14	1.451 ±0.09	77.4%*	0.022
% Image Area Occupied by ChAT+ Dendrites	7.673 ±0.61%	5.186 ±0.37%	67.6%*	0.003
Morphological Parameter – VGLUT2+ Terminals on ChAT+ Dendrites	WT Mean (±SEM)	Q140 Mean (±SEM)	Q140 as Percent of WT	P Value *
Abundance of VGLUT2+ Synaptic Terminals Contacting ChAT+ Dendrites (per μm2)	0.00273 ±0.0003	0.00176 ±0.0002	64.5%*	0.013
Abundance of VGLUT2+ Synaptic Terminals per ChAT+ Dendrite Unit Area (μm2)	0.0385 ±0.006	0.0363 ±0.005	94.3%	0.781

^*Significant differences between WT and Q140 mice are shown in bold in the last column.