Skip to main content
. 2016 Jun 21;40(10):1582–1590. doi: 10.1038/ijo.2016.96

Figure 1.

Figure 1

HFD results in decreased lymphatic vascular density in obesity-prone mice. (a) Representative H&E-stained sections of back skin from NCD- or HFD-fed C57BL/6J, BALB/cJ and MSTNln mice (scale bar=200 μm; bracket surrounds subcutaneous adipose tissues). (b) Representative immunofluorescent localization of LYVE-1+ vessels in upper limb tissues of NCD- or HFD-fed mice in all groups (scale bar=100 μm). (c) Upper panel: Body weights of mice on NCD (open circles) and HFD (filled circles) in all groups (n=5/group). C57BL/6J HFD vs NCD (*P<0.0001). BALB/cJ HFD vs NCD (*P<0.05), MSTNln mice had no significant difference. Middle panel: Quantification of subcutaneous soft tissue thickness in NCD- and HFD-fed mice in all groups (n=5–10/group). C57BL/6J HFD vs NCD (*P<0.0001). MSTNln and BALB/cJ mice show no significant difference between NCD and HFD groups. Lower panel: Quantification of upper limb LYVE-1+ lymphatic vessel density per high powered field (HPF) and quadrant in NCD- and HFD-fed mice in all groups (n=5 animals × 4HPF/group). C57BL/6J HFD vs NCD (*P<0.0001). MSTNln and BALB/cJ mice show no significant difference between NCD and HFD groups.