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. Author manuscript; available in PMC: 2017 Oct 4.
Published in final edited form as: Chembiochem. 2016 Jul 29;17(19):1800–1803. doi: 10.1002/cbic.201600213

Figure 2.

Figure 2

(A) HeLa were transfected with HA-tagged HRas (wt and C181,184S mutant) for 24h. 4h before lysis, cells were treated with alkyne-tagged fatty acid, immunoprecipitated, and reacted with az-rhodamine. Chemical reporters (alk-16:1, alk-17:1, alk-18:1) label HA-HRas on key Cys residues. Anti-HA western blot is included as a loading control. (B) In-gel fluorescence analysis of −/+ LPS/IFN-γ stimulated Raw264.7 treated with oleic acid (18:1), alk-16, alk-16:1, alk-17:1, alk-18:1 (50 μM, 4 h). Coomassie staining was included as a loading control.