BEAS-2B cells incubated in antibiotic-free and FBS-free culture medium with A) NM MOI 10, 2h, 4h and 24h. Adherent bacteria are reported as CFU/well ± SEM. **p = 0.004 and 0.0021; ****p < 0.0001 by Mann Whitney test; B) NL MOI 10, 2h, 4h and 24h. ***p = 0.0004 and ****p < 0.0001 as above; C) NM (gray bars) and NL (white bars), MOIs of 10 and 100, 2h. **p = 0.0028 and ***p = 0.0007 as above. D) Intracellular NM (gray bars) and NL (white bars), MOIs of 10 and 100, 2h by gentamicin assay (100 μg/ml, 1h) reported as CFU/well ± SEM. ***p = 0.0004 and *p = 0.043 as above. E) Intracellular NM (gray bar) and NL (white bar), MOI 100, 2h, following cytochalasin D (CytD) treatment (5 μg/ml, 1h). Intracellular bacteria are reported as percent relative to non CytD-treated cells (dashed line) ± SEM. ***p = 0.0007 and ****p < 0.0001 by one sample t test; p = n.s. by Mann Whitney test.