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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Microbes Infect. 2016 Jul 1;18(10):627–638. doi: 10.1016/j.micinf.2016.06.001

Fig. 2. PorB and TLR2-dependent Neisseriae internalization in BEAS-2B cells.

Fig. 2

A) BEAS-2B cells were incubated in antibiotic-free and FBS-free culture medium with NM-[Nlac PorB] MOI 100, 2h. Intracellular bacteria are expressed as percent of recovered intracellular NM (gray dotted bar and dashed line, respectively), or NL (white dotted bar and dashed line, respectively) ± SEM. *p = 0.02 and ****p < 0.0001 by one sample t test. B) Intracellular NM-[Nlac PorB] (striped bar), NM (gray bar) and NL (white bar) (MOI 100, 2h) recovered following cell stimulation with purified recombinant PorB (PorBWT) (10 μg/ml, 24h) and expressed as percent of non PorBWT-stimulated cells (dashed line) ± SEM.**p = 0.007; ***p = 0.0002 and 0.0001 by one sample t test. Among strains, p = n. s. by Mann Whitney test. C) Intracellular bacteria as above following stimulation with Pam3CSK4 (200 ng/ml, 24h). *p = 0.015; ***p = 0.0008 and 0.0003 as above. Among strains, *p = 0.016 and ***p = 0.0009 as above. D) Intracellular bacteria as above following stimulation with PorBDDE/AKR (10 μg/ml, 24h). **p = 0.0017 and ****p < 0.0001 as above. Among strains, p = n. s. as above.