Skip to main content
. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Microbes Infect. 2016 Jul 1;18(10):627–638. doi: 10.1016/j.micinf.2016.06.001

Fig. 3. Neisseriae internalization by HEK cells.

Fig. 3

A) pcDNA and TLR2 HEK cells incubated in antibiotic-free culture medium with NM-[Nlac PorB] (striped bar), NM (gray bar) and NL (white bar), MOI 100, 2h. Intracellular bacteria are reported as percent of intracellular organisms from TLR2 HEK cells relative to pcDNA HEK cells (dashed line) ± SEM. **p = 0.0062 and ***p = 0.0001 and 0.0002 by one sample t test. Among strains, ****p < 0.0001 by Mann Whitney test. B) Intracellular NM-[Nlac PorB] (striped bar), NM (gray bar) and NL (white bar), MOI 100, 2h in TLR2 HEK cells following cell stimulation with purified recombinant PorB (PorBWT) (10 μg/ml, 24h) expressed as percent of non PorBWT-stimulated cells (dashed line) ± SEM. *** p = 0.0007 and ****p < 0.0001 by one sample t test as above. Among strains, **p = 0.0061, ***p = 0.0001 and ****p< 0.0001 by Mann Whitney test as above. C) Intracellular bacteria as above following stimulation with Pam3CSK4 (200 ng/ml, 24h). **p = 0.0063 by one sample t test as above. Among strains, p = n. s. as above. D) Intracellular bacteria as above following stimulation with PorBDDE/AKR (10 μg/ml, 24h). **p = 0.0028 and 0.0098 as above. Among strains, *p = 0.024 as above.