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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Cancer Res. 2016 Aug 16;76(19):5671–5682. doi: 10.1158/0008-5472.CAN-16-0144

Figure 4. GL261 tumors are infiltrated by CCR4-expressing Tregs and CCR2-expressing MDSCs.

Figure 4

(A–B) Flow cytometric quantification of CCR4 and CCR2 expression among CD4+Foxp3− T cells, CD4+Foxp3+ Tregs, and CD8+ T cells (n = 5). (C–D) Quantification of CCR4 and CCR2 expression on T cell subsets in brain compared to cervical lymph nodes and spleen (E–F) Absolute numbers of CD11b+Ly-6G+ and CD11b+Ly-6C+ cells in the brains of tumor bearing or PBS-injected mice (n = 5). (G) CCR4 and CCR2 expression in MDSC subsets and microglia. (H) Quantification of frequencies in G (n = 5). Data are representative of at least 4 independent experiments. Data are represented as mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 by ordinary one-way ANOVA with Tukey’s multiple comparisons test in B, D, and H or Student’s t test in E–F. cLN, cervical lymph nodes. Sp, spleen.