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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Mol Cancer Ther. 2016 Aug 2;15(10):2294–2301. doi: 10.1158/1535-7163.MCT-16-0153

Figure 3. Overexpression of SHP inhibits HCC cell invasion and migration.

Figure 3

(A) Comparing the migration potential in Hep3B, Huh7, HMCC97L and MHCC97H cells. Migrated cells were visualized by the staining of crystal violet.

(B) Left: The levels of secreted CCL2 protein in culture medium as examined by ELISA. Right: Semi-quantitative PCR of CCL2 mRNA in various HCC cells.

(C) HCC cell migration assay. Huh7 (left) and Hepa1 (middle) cells were overexpressed Ade-SHP (adenovirus, 50 MOI) for 48 hrs. Data is represented as mean ± SE of triplicate assays (*p<0.01 vs. white bar). Right: Images of invaded cells stained with crystal violet.

(D) HCC cell migration assay. MHCC97H cells were transduced with Ade-SHP (50 MOI) for 48 hrs and migrated cells were visualized by the staining of crystal violet. Data are representative of two or more experiments.