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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Mol Cancer Ther. 2016 Aug 2;15(10):2294–2301. doi: 10.1158/1535-7163.MCT-16-0153

Figure 4. Activation of SHP inhibits CCl2-mediated HCC cell invasion and migration.

Figure 4

(A) HCC invasion assay. MHCC97H cells were transduced with Ade-SHP for 48 hrs before subjected to invasion assay in the absence or presence of CCL2 (50 ng/ml). The invaded cells were stained with crystal violet and counted. Quantitative results on the right: *p<0.01 vs. GFP (-CCL2); ¥p<0.01 vs. GFP (+CCL2).

(B) HCC migration assay. Huh7 cells were transduced with indicated adenovirus for 48 hrs before subjected to migration assay in the absence or presence of CCL2 (100 ng/ml). The migrated cells were stained with crystal violet and counted. *p<0.01 vs. GFP (-CCL2); ¥p<0.01 vs. GFP (+CCL2).

(C) HCC migration assay. DSHN group: 50 μM for 48 hrs; CCL2 group: 50 ng/ml for 24 hrs. The migrated cells were stained with crystal violet and counted. Quantitative results on the right: *p<0.05 vs. DMSO (-CCL2); ¥p<0.01 vs. DMSO (+CCL2) and DSHN (-CCL2).

(D) HCC migration assay in Huh7 cells treated with DSHN (50 μM). *p<0.01 vs. DMSO. Data are representative of two or more experiments.