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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Cancer Res. 2016 Aug 12;76(19):5615–5627. doi: 10.1158/0008-5472.CAN-15-1824

Figure 2. The lncRNA LUST overexpression is an early event in the spheres formation process.

Figure 2

A) LUST, CD24 and CD44 mRNA levels measured by qRT-PCR in HT-29 derived spheres formed with colon carcinoma cells, respectively after 14 and 35 days cultured in ultra-low attachment conditions and in FBS-induced differentiation cells. HPRT transcript was used as housekeeping control gene for normalization. Error bars indicate the standard error of the mean (SEM). *p≤0.05; B) FACS analysis of spheres formed with colon adenocarcinoma cell lines (upper panels) and FBS-induced differentiated cells (lower panels) showing corresponding loss of expression of stem-like markers (blue population). Histograms report the mean fluorescence intensity (MFI, range 0–10,000) of CD24 (FITC) and CD44 (PE-Cy7); C) Representative images of HT-29-derived spheres formed with colon adenocarcinoma cells with characteristic spheroid morphology (top) and differentiated spheres showing adherent morphology upon FBS-induced differentiation (bottom).