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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Cancer Res. 2016 Aug 12;76(19):5615–5627. doi: 10.1158/0008-5472.CAN-15-1824

Figure 4. LUST expression promotes spheroid formation across multiple human colon adenocarcinoma cell lines.

Figure 4

A) WB analysis of total cellularβ-catenin, active β-catenin, c-Myc and Cyclin D1 in LS174T control cells and LUST overexpressing cells, α-tubulin was used as a loading control. B) qRT-PCR validation of WNT-target genes expression upon LUST overexpression, C) Representative images of colonospheres formed with colon cancer cells derived from pcDNA3, and pcDNA3-LUST transfected cells. D) Relative mRNA levels of WNT-target genes upon LUST overexpression in SW-480 colon cancer cells, analyzed by qRT-PCR. E–G), Representative images of spheres formed respectively with SW-480, DLD-1 and HT116 colon cancer cells, derived from pcDNA3 and pcDNA3-LUST transfected cells. Cells were cultured in “ultra-low” attachment conditions, in serum free media for 7, 14 and 21 days. For qRT-PCR experiments HPRT was used as housekeeping control gene for normalization. Error bars indicate the standard error of the mean (SEM). *p≤0.05.