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Autophosphorylation Rate Constants for CheY T+1 Mutants
| Amino acid at T+1 | kphos/KS (M−1s−1)a
|
||
|---|---|---|---|
| PAM | AcP | MPI | |
| Ser | 10 ± 0.6 | 4.5 ± 0.8 | 15 ± 5 |
| Thr | 4.6 ± 0.5 | 4.2 ± 0.3 | 3.9 ± 0.9 |
| Ala | 9.2 ± 0.9 | 9.8 ± 2 | 54 ± 10 |
| Met | 3.1 ± 0.4 | 8.9 ± 2 | 26 ± 4 |
| Val | 0.34 ± 0.06 | 1.9 ± 0.03 | 3.7 ± 0.04 |
| Ile | 0.31 ± 0.06 | 1.6 ± 0.3 | 3.7 ± 0.2 |
Mean and standard deviations of the rate constants were determined from individual autophosphorylation time courses. Rate constants for wild type CheY are underlined.
