Table 6.
Phosphotransfera Between Partner and Non-Partner Sensor Kinases and Response Regulators
| Response Regulator | Amino Acid at Position T+1 | Sensor Kinase
|
|
|---|---|---|---|
| CheA | MBP-NarX227 | ||
| CheB | Ser (wild type) | + | − |
| Ala | + | − | |
| Val | +/− | − | |
| CheY | Ser | + | − |
| Thr | + | − | |
| Ala (wild type) | + | + | |
| Met | + | + | |
| Ile | + | + | |
| Val | + | + | |
| NarL | Ser | +/− | +/− |
| Ala | − | + | |
| Val (wild type) | − | + | |
a
The manual phosphotransfer assay is relatively insensitive; i.e. only very large reductions in phosphotransfer rate can be detected. Symbols: +, phosphotransfer indistinguishable from wild type partner; +/−, reduced phosphotransfer; −, no detectable phosphotransfer.