Fig. 2.

Release of insert from pET 28a vector with restriction enzymes. Recombinant vector and empty vector were incubated with or without restriction enzymes used for cloning for 2 h at 37 °C. The products were separated on 1 % agarose gel. Lane 1: 1 kb marker; Lane 2: pET28a vector without enzymes; Lane 3: pET28a vector with restriction enzymes; Lane 4: Recombinant pET28a without restriction digestion; Lane 5: Recombinant pET28a vector with restriction digestion