Figure 1.

Overview of S. cerevisiae metabolic pathways relevant to this study and metabolic interventions. A non-native ATP citrate lyase (ACL) was introduced with the intention of increasing acetyl-CoA (accoa) supply, but fatty acid production did not increase significantly (Figure 2). The use of flux analysis suggested that more acetyl-coA was indeed produced, but that it was lost through malate synthesis (MLS, Figure 3). Downregulating this enzyme increased fatty acid (FA) production 26% (Figure 2). The use again of flux analysis suggested knocking out glycerol production (GPD1) for increased production. FA production improved an extra 33% (Figure 2). The mitochondrial and peroxisomal compartments are represented as blue and yellow circles, respectively. Metabolite abbreviations follow the BIGG database (Schellenberger et al., 2010).