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. 2016 Oct 5;4:76. doi: 10.3389/fbioe.2016.00076

Figure 4.

Figure 4

Cytosolic acetyl-CoA balances obtained from 2S-13C MFA for strains with GPD1 knocked out. Flux of acetyl-CoA (accoa) producing and consuming reactions is shown as a sankey diagram as in Figure 3. In spite of the large confidence intervals for the calculated fluxes, several conclusions can be drawn. The total flux through ACCOACr, for example, is doubled through the full engineering process presented here: compare [0.49–0.83] for WRY2 in Figure 3 versus [1.43–3.39] for WRY2 ΔGPD1 ACL PTEF1-MLS1 in this figure. Also, the effect of the GPD1 knockout definitely increases flux through ACCOACr ([0.49–0.83] for WRY2 in Figure 3 versus [0.97–4.38] for WRY2 ΔGPD1). Furthermore, the effect of ACL involves the definite activation of reaction MALS as a carbon sink (similar effect as in Figure 3).