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. 2016 Oct 5;7:452. doi: 10.3389/fphys.2016.00452

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Copyright © 2016 Wu, Lin, Zhang and Zeng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Recombinant BdorOBPs and a BdorCSP analyzed on SDS-PAGE. M, Molecular weight marker; 1, Total protein extract from non-induced pET32a transformed BL21 (DE3) cells; 2, Protein extract from isolated inclusion body of pET32a transformed cells; 3, Supernatant of ultrasonated pET32a transformed cells; 4, Proteins purified through Ni-NTA columns; 5, Purified proteins with His-tag cleaved using recombinant enterrokinase; (A), BdorCSP3; (B), BdorOBP56h; (C), BdorOBP83a-1; (D), BdorOBP83a-2; (E), BdorOBP84a-1; (F), BdorOBP84a-2.