Figure 3.

Susceptibility tests of N. crassa to ketoconazole, the Hsp90 inhibitor geldanamycin and the two drugs combined. (A) Susceptibility tests with geldanamycin (GA, 2 mg/L), ketoconazole (KTC, 1 mg/L) or the two drugs combined. Two microliters of conidial suspension (2 × 10⁶ conidia/ml) were inoculated in the center of plates (Φ 90 mm) with or without antifungal drugs or oxidants, and then incubated at 28 or 42°C (heat tests) for the indicated time. (B) Relative growth inhibition rates were calculated based on colony diameters at 24 h after drug treatment. Values from three replicates were used for a statistical analysis. Means of the inhibition rates are shown, and standard deviations are marked with error bars. Differences between the mutants and the WT were statistically analyzed by the analysis of variance. Values with P < 0.0001, 0.0001 < P < 0.001, 0.001 < P < 0.01, and 0.01 < P < 0.05 are marked with ^****, ^***, ^** and ^*, respectively.