Figure 4.

Differential expressions of genes in the Δsti1, Δaha1, and Δp23 strains relative to the wild type (WT) strain was determined by quantitative RT-PCR. Strains were grown in liquid Vogel's medium at 28°C with shaking at 180 rpm for 12 h. Then ketoconazole (KTC) was added to the medium to reach 2.5 mg/L. After 24 h incubation, the following genes were analyzed by quantitative RT-PCR: hsp80, erg11, erg6, and cdr4. Values shown are the means of three independent replicates. Standard deviations are indicated by error bars, and differences between the mutants and WT were statistically analyzed by analysis of variance. Values with P < 0.0001, 0.0001 < P < 0.001, 0.001 < P < 0.01, and 0.01 < P < 0.05 are marked with ^****, ^***, ^** and ^*, respectively.