Figure 11.

Nrg1 is required in neurons for Pacinian corpuscle formation. Ret^CreERT2/+;Nrg1^f/+ controls and Ret^CreERT2/+;Nrg1^f/− mutants were treated with tamoxifen at E13.5 and E14.5 to ablate Nrg1 conditional allele in Ret⁺ cells, including Pacinian corpuscle-innervating neurons. A, B, Anti-S100 (red) and anti-NFH (green) staining of P5 hindlimb sections shows a lack of S100⁺ Pacinian corpuscles in the Nrg1 conditional mutant mice. C, D, H&E-stained hindlimb sections show a lack of Pacinian corpuscle-like Schwann cell structures in Nrg1 conditional mutant mice. E, F, S100/NFH staining of hindlimb sections proximal to the appearance of Pacinian corpuscles around the fibula shows axons of the interosseous nerve, which innervate Pacinian corpuscles, present in both mutant and control mice. N = 3 animals, 6 legs per genotype. Scale bar, 20 μm.