Figure 15.

Schwann cell-derived Nrg1 is not required for maintenance of Pacinian corpuscle following denervation. A–D, Two- to 4-month-old Dhh^Cre;Nrg1^f/+ control (A, C) and Dhh^Cre;Nrg1^f/f conditional mutants (B, D) underwent unilateral tibial nerve transection and recovered for 3 months before tissue processing. NFH⁺ (green) innervating fibers were present in the S100⁺ (red) corpuscle inner cores of cross-sections of nonsurgical limbs (A, B) but were absent from corpuscle inner cores of denervated limbs (C, D). E, Quantification of S100⁺ Pacinian corpuscles in serial hindlimb sections in Dhh^Cre;Nrg1^f/f controls (38.33 ± 1.86 corpuscles per nonsurgical limb, 40.33 ± 2.73 corpuscles per denervated limb; p = 0.58) and Dhh^Cre;Nrg1^f/f conditional mutants (36.33 ± 0.88 corpuscles per nonsurgical limb, 38.67 ± 0.67 corpuscles per denervated limb; p = 0.10) shows that loss of Nrg1 in Schwann cells does not cause a deficit in the maintenance of Pacinian corpuscles following denervating injury. N = 3 legs per condition. Not significant: p ≥ 0.05. Error bars indicate SEM. Scale bar, 50 μm.