Figure 3.

T₃ increases tectal NPC proliferation. A, Protocol for T₃ treatments, followed by immunohistochemical (IHC) labeling for pH3. Stage 47 tadpoles were put into either control bath or T₃ bath (50 nm) for 7 d. Tadpoles from both groups were killed 2, 4, and 7 d after onset of treatment. B, Representative confocal z-projections of optic tecta immunolabeled for pH3 (green) and counterstained with Sytox-O (red). C, Treatment with T₃ significantly increased the number of pH3+ cells in the ventricular zone at all three time points (**p < 0.01, ***p < 0.001). n = 10–14 brains per group.