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METH or PMA internalizes α subunits, while BIM-1 inhibition of PKC prevented internalization of the GFP-α subunit. A, Representative TIRF microscopy images of the GFP-α subunit at the plasma membrane of HEK 293 cells following vehicle, 10 μm METH, 1 μm BIM-1 plus METH, or 0.1 μm PMA treatment. B, C, Data are mean ± SEM; analyses of relative fluorescent intensities at the surface membrane following vehicle, METH, METH plus BMI-1 or PMA treatments. N = 5 or 7 per group.
