TABLE 2 .
Location of lasR sequence variations in isolates with residual LasR activitya
| Isolate | Nucleotidechange | Amino acidchange | Relative activityin P. aeruginosab | Relative activityin E. colic |
|---|---|---|---|---|
| E32 | C649T | R217W | 21 ± 2 | 5 ± 1 |
| E34 | C292T | Q98*d | NDe | 130 ± 32 |
| E50 | C564A | C188* | 80 ± 1 | 65 ± 19 |
| E54 | C350T | P117L | 50 ± 8 | 98 ± 25 |
| E100 | A641G | N214S | ND | 119 ± 21 |
| E106 | C239T | T80I | 77 ± 27 | 3 ± 1 |
| E145 | C219G | D73E | 51 ± 2 | 81 ± 8 |
| E183 | C478T | Q160* | 136 ± 4 | 138 ± 22 |
LasR activity was measured in P. aeruginosa EPIC isolates and/or in recombinant E. coli containing lasR genes from the indicated isolates, as described in Materials and Methods. The following LasR variants showed no activity in the E. coli reporter assay: E33 (G61A), E37 (G588T), E41 (G31T), E42 (G671A), E56 (G338A), E57 (G307T), E58 (C692T), E59 (A625G), E67 (G179A), E68 (T227G), E89 (G543C), E92 (G490T), E104 (A532G), E108 (C355T), E113 (T55C), E129 (C149T), E130 (T662A), E131 (A580G), E151 (G199C), E165 (G703A), E186 (C314T), and E188 (T452C).
Percent activity (± standard deviation) in comparison to the laboratory strain PAO1.
Percent activity (± standard deviation) in comparison to E. coli with the P. aeruginosa PAO1 lasR gene.
An asterisk indicates a stop codon.
ND, not determined (unable to transform clinical isolate with plasmid).