FIG 5 .

Grasp65 is phosphorylated during HCMV infection. HF cells were plated and grown to confluence prior to serum starvation for 72 h to synchronize cells. Cultures were infected with HCMV AD 169 at an MOI of 1. (A) Steady-state levels of Grasp55 are increased by HCMV infection compared to the level in an uninfected, asynchronous population (cycling) or uninfected, noncycling cells (confluent). (B) Grasp65 levels increase during infection, and higher-molecular-weight forms can be detected in cycling and infected cells. (C) HCMV infection induces higher-molecular-weight forms of Grasp65 that are eliminated with calf intestinal phosphatase (CIP) treatment but not by incubation in a cocktail of phosphatase inhibitors (PIC). Numbers to the left of each gel show molecular weight (in thousands).