FIG 7 .

Grasp65-7A–GFP mutant is hypophosphorylated compared to Grasp65wt-GFP in HCMV-infected cells. HF cells were transduced with recombinant lentiviruses expressing either Grasp65wt-GFP or Grasp65-7A–GFP and synchronized prior to infection with HCMV AD 169 at an MOI of 1. Cultures were harvested on day 3 and 4 postinfection, and lysates analyzed for Grasp65wt or Grasp65-7A GFP-tagged protein as described in the legend to Fig. 5. Treatment of infected cell lysates with calf intestinal phosphatase (CIP +) increased the migration of higher-molecular-weight species of Grasp65wt-GFP but did not alter the migration of Grasp65-7A–GFP, suggesting that the Grasp65-7A–GFP mutant is hypophosphorylated during infection compared to Grasp65wt-GFP.