Table 3. EC50 values of JFH1/5ACon1 and its derivatives with resistance-associated NS5A mutations to SMV, ASV and SOF.
| Strain (JFH1/5ACon1-) | SMV |
ASV |
SOF |
|||
|---|---|---|---|---|---|---|
| EC50 (nM) (95% CI) | Fold Resistance | EC50 (nM) (95% CI) | Fold Resistance | EC50 (nM) (95% CI) | Fold Resistance | |
| wt | 173 (142–210) | 1.00 | 664 (541–814) | 1.00 | 539 (465–624) | 1.00 |
| L31M | 187 (137–237) | 1.08 | 684 (503– 870) | 1.03 | 503 (404–604) | 0.934 |
| L31V | 158 (117–200) | 0.915 | 717 (533–903) | 1.08 | 303 (244–363) | 0.563 |
| L31I | 197 (146–247) | 1.14 | 633 (456–810) | 0.953 | 582 (466–695) | 1.08 |
| Y93H | 110 (87.0–133) | 0.638 | 291 (221–361) | 0.438 | 663 (530–792) | 1.23 |
| L31M/Y93H | 101 (79.6–122) | 0.582 | 282 (214–350) | 0.425 | 614 (493–733) | 1.14 |
| L31V/Y93H | 118 (92.4–143) | 0.681 | 374 (275–473) | 0.563 | 561 (450–674) | 1.04 |
| L31I/Y93H | 119 (92.9–144) | 0.686 | 375 (276–475) | 0.565 | 647 (521–776) | 1.20 |
SMV; Simeprevir, ASV; Asunaprevir, SOF; Sofosbuvir, EC50; effective concentrations required to inhibit 50% of intracellular core protein level, CI; confidence interval.