Skip to main content
. 2016 Oct 5;6:34624. doi: 10.1038/srep34624

Figure 3. GDF11/8 localization in the kidney and expression pattern during AKI.

Figure 3

(a,b) Immunofluorescence was performed using a GDF11 antibody (ab124721) on normal kidney sections of 3-month-old C57Bl/6 mice. GDF11 was mainly expressed in the proximal tubular cells (confocal with LTL) and slightly expressed in the mesangial cells (confocal with α-SMA). Magnification, 600×. (c,d,f) GDF11 mRNA (c) and protein (d,e) expression in the kidneys of 3-month-old and 24-month-old C57Bl/6 mice (each lane represents a separate mouse). (e,g) The mature GDF11 peptide increased in the kidneys of 3-month-old and 24-month-old C57Bl/6 mice that underwent IRI (each lane represents a separate mouse). For GDF11 mRNA (c), data are means ± SD, n = 6 in each group, ##P < 0.01 vs. young mice. For GDF11 protein in the kidneys of young and old mice (f), data are means ± SD, n = 3 in each group, #P < 0.05 vs. young mice, ##P < 0.01 vs. young mice. For GDF11 protein in the kidneys of young and old mice that underwent IRI (g), data are means ± SD, n = 4 in each group, *P < 0.05 vs. Sham, **P < 0.01 vs. Sham, #P < 0.05 vs. young mice with 35 min of IRI, ##P < 0.01 vs. young mice with 35 min of IRI, $P < 0.05 vs. IRI at 48 h after IRI.