Table 1. The ratio of each protein was determined as the ratio between the percentage of phosphorylated proteins to total proteins in GDF11 or 0.1% BSA (control) treatment.
| Phosphorylation sites | Ratio 1 (GDF11/NC) | Ratio 2 (GDF11/NC) | Mean ratio (GDF11/NC) |
|---|---|---|---|
| Abl1 (Phospho-Thr754/735) | 1.61 | 1.58 | 1.60 |
| AKT (Phospho-Ser473) | 2.03 | 1.31 | 1.67 |
| ERK1-p44/42 (Phospho-Thr202) | 2.61 | 2.66 | 2.64 |
| Myc (Phospho-Ser62) | 2.66 | 2.77 | 2.72 |
| Smad1 (Phospho-Ser187) | 2.16 | 2.11 | 2.14 |
| Smad2 (Phospho-Ser250) | 1.59 | 1.58 | 1.59 |
| PKC theta (Phospho-Thr538) | 0.34 | 0.11 | 0.23 |
Mean values from two independent experiments are shown.