Figure 3. Bladder functional protein and contractility assessments.

(a–c) representative blots and changes in average functional protein expression in the bladder of adult offspring assessed by Western blot analysis with specific antibodies to M2 and M3-mAChRs, purinergic P2X₁ receptor and GAPDH. Full-length blots/gels are presented in Supplementary Fig. S2. Experiments were repeated two times and representative blots are shown (upper panels). Data of proteins expression (ratios of signal intensities of investigated proteins relative to GAPDH) were calculated with 8 samples in each group. Data which were standardized and expressed in percentage to the control group (lower panels) (d–e) The concentration-response curves (n = 6, in each group) of the denuded detrusor muscle in response to the treatment with different doses of KCl, carbachol or ATP. In each experiment contractile response was expressed as percent of 300 mM KCl-induced mean contractility of the control group. All data are presented in Mean ± SE. An asterisk indicates a significant difference between controls and the MFE group (Two samples t test, p < 0.05).