Table 1. Comparative analysis of carbonic anhydrase putative active site pocket amino acid residues and amino acids whose side chains either project into or border the active site in lamprey (Pma) Ca18 (GenBank;.AAZ83742.1) and Ca19 (GenBank, ALM25804.1), with rainbow trout (Omy) Cac (Ca17a) (GenBank, NP_001166020.1), and human (Hsa) CA1 (GenBank, NP_001729.1) and 2 (GenBank, NP_000058.1) modelled after Tashian et al. ⁴ and Gilmour et al. ¹⁵.

	7	29	61	62	64	65	66	67	69	91	92	94	96	106	107	117	119	121	131	141	143	145	192	194	198	199	200	201	202	204	206	207	209	211	244	246
	*	*			*			*			*	*	*	*	*	*	*							*		*	*						*		*	*
					+							Z	Z				Z	~		~	~				~							~	~
Hsa CA2	Y	S	N	N	H	S	F	N	E	I	Q	H	H	E	H	E	H	V	F	L	V	G	W	Y	L	T	T	P	P	L	C	V	W	V	N	R
Pma Ca19	.	.	.	S	.	.	.	S	D	G	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	N	S	A	.	.	T	P
Pma Ca18	.	.	.	.	.	.	.	S	.	K	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	F	S	.	.	.	.	.
Omy Ca17a	.	.	.	.	.	.	.	Q	T	K	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	S	.	.	.	.	.
Hsa CA1	.	.	.	V	.	.	.	H	N	F	.	.	.	.	.	.	.	A	L	.	.	.	.	.	.	.	H	.	.	Y	S	.	.	I	.	.

The aa were aligned using ClustalW (BioEdit 7.0.9.0). Numbers represent the location of each amino acid relative to alignment with human CA2. *Active site aa residues; Z, zinc binding ligand; + , proton shuttling associated ligand; ~, substrate associated pocket.