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. 2016 Oct 5;6:34215. doi: 10.1038/srep34215

Figure 2. T cell responses to DEN2-80E in mice.

Figure 2

2 weeks after final vaccine dose, spleens from immunized mice were collected, processed, and total DEN2-80E specific T cell response was evaluated. IFN-γ producing spots per million (SFU) splenocytes were determined by IFN-γ ELISPOT assay. Bars represent average value from pooled spleens from 5 randomly selected mice out of 10 immunized mice/group, after background subtraction from non-stimulated cells (a). DEN2-80E specific CD4+ T cells that express IFN-γ, and TNF-α (b), and DEN2-80E specific CD8+ T cells that express IFN-γ, and TNF-α (c) were evaluated by intracellular FACS assay (FACS ICS). Bars represent T-cell events per million splenocytes from 5 randomly selected mice out of 10 immunized mice/group after background subtraction from unstimulated cells.