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. 2016 Oct 5;6:34215. doi: 10.1038/srep34215

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The immunization regime, dosing schedule, and vaccine formulation details for Guinea pigs vaccinated with tetravalent Dengue vaccine (Tetra DEN-80E) is depicted (a,b). Dengue virus serotype 1 (D1), serotype 2 (D2), serotype 3 (D3), serotype 4 (D4) specific neutralizing activity from individual immunized Guinea pig sera was calculated using the LiCor based assay and depicted as LiCor microtneut NT50 titers (c). Each dot/symbol represents an individual animal. The geometric mean titers (GMT) of serotype specific neutralization are depicted on top of the graph above each group. DEN1 titers: *p < 0.001 as compared to DEN80E + PBS group; DEN2 titers: **p < 0.001 as compared to DEN80E + PBS group; DEN3 titers: ***p < 0.001 as compared to DEN80E + PBS group; DEN4 titers: ****p < 0.001 as compared to DEN80E + PBS group.