Table 2. Identification of differentially expressed proteins between control A. lancea plantlets and plantlets induced by AL12 exopolysaccharides.
Putative function | Spot no.a | Protein | Gene or gene locus | Mascot score | Protein ID | Mr (Da)b | pIb | Fold changec | p-value |
---|---|---|---|---|---|---|---|---|---|
Photosynthesis | 4 | hypothetical protein PHAVU_007G155500g | PHAVU_007G155500g | 167 | gi|593687543 | 14047 | 9.60 | 2.87 | 0.031 |
6 | chlorophyll a/b-binding protein CP26 precursor | LOC_Os11g13890 | 152 | gi|62733870 | 24317 | 5.95 | 4.35 | 0.029 | |
14 | chlorophyll a/b binding protein precursor | AF220527 | 162 | gi|6716783 | 28542 | 5.29 | 2.58 | 0.047 | |
17 | chlorophyll a/b binding protein | CAB1 | 238 | gi|5714656 | 28613 | 5.29 | 3.21 | 0.023 | |
21 | chlorophyll a-b binding protein 8 | LOC100232927 | 158 | gi|225436257 | 29511 | 7.85 | 2.06 | 0.036 | |
35 | carbonic anhydrase | FBU08398 | 136 | gi|40737972 | 36025 | 5.85 | 7.98 | 0.041 | |
37 | ribulose bisphosphate carboxylase large chain | rbcL | 305 | gi|131899 | 52159 | 6.13 | 10.12 | 0.040 | |
58 | ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, partial | rbcL | 199 | gi|1881503 | 52231 | 6.03 | 3.51 | 0.019 | |
59 | ribulose-1,5-bisphosphate carboxylase, partial | rbcL | 188 | gi|2961313 | 53408 | 6.05 | 2.01 | 0.035 | |
Oxidation-reduction, oxidative burst | 2 | hypothetical protein GUITHDRAFT_152572, NADH-ubiquinone reductase complex 1 MLRQ subunit | GUITHDRAFT_152572 | 84 | gi|551660038 | 12249 | 9.95 | 10.29 | 0.021 |
20 | putative protein, NADPH-dependent FMN reductase | AT4g27270 | 64 | gi|3269288 | 22355 | 6.30 | 4.32 | 0.048 | |
24 | oxygen-evolving enhancer protein 2 | LOC103990328 | 146 | gi|695040260 | 28178 | 8.61 | 2.02 | 0.022 | |
25 | photosystem II oxygen-evolving enhancer protein 2 | GSCOC_T00013178001 | 258 | gi|661878401 | 28714 | 8.22 | 2.14 | 0.031 | |
30 | N-ethylmaleimide reductase | FadH | 74 | gi|659891198 | 39341 | 4.94 | 2.16 | 0.037 | |
34 | monooxygenase | AT4G38540 | 69 | gi|3426064 | 45505 | 5.94 | 2.53 | 0.020 | |
42 | NAD-dependent epimerase/dehydratase | MtrDRAFT_AC155890g8v2 | 81 | gi|124360315 | 31765 | 7.64 | 2.23 | 0.034 | |
45 | NADPH: adrenodoxin | NGA_0622900 | 84 | gi|553192841 | 153673 | 8.64 | 0.47 | 0.028 | |
46 | gamma-glutamyl putrescine oxidoreductase, putative, partial | RCOM_0368500 | 82 | gi|223524108 | 29290 | 7.85 | 2.28 | 0.029 | |
47 | oxygen-evolving enhancer protein 1 | PSBO | 190 | gi|131385 | 35595 | 5.84 | 3.74 | 0.019 | |
48 | hypothetical protein BCCGELA001_06795, partial, COG1032 Fe-S oxidoreductase | BCCGELA001_06795 | 70 | gi|404270905 | 35764 | 6.13 | 3.62 | 0.027 | |
55 | NADP-dependent oxidoreductase P1 | LOC100282970 | 88 | gi|226528403 | 38936 | 6.22 | 2.29 | 0.024 | |
Energy metabolism | 8 | adenylate kinase B | ADK-B | 72 | gi|728811055 | 27013 | 6.45 | 2.44 | 0.012 |
13 | ATP synthase delta chain | LOC104229705 | 230 | gi|698456786 | 26776 | 8.94 | 3.93 | 0.044 | |
18 | butyrate kinase | PRK03011 | 84 | gi|499737230 | 39210 | 5.81 | 0.42 | 0.045 | |
19 | protein phosphatase 2A catalytic subunit | PP2Ac2 | 74 | gi|350537893 | 35010 | 4.78 | 2.11 | 0.039 | |
32 | phosphoglycerate kinase | LOC100249576 | 111 | gi|225464995 | 50166 | 8.26 | 8.20 | 0.025 | |
33 | ATP synthase gamma chain | LOC103446721 | 135 | gi|657983988 | 41501 | 7.52 | 3.33 | 0.031 | |
52 | V-type proton ATPase catalytic subunit A | AT1G78900 | 94 | gi|332198050 | 69077 | 5.29 | 10.58 | 0.038 | |
53 | ATP-dependent Clp protease | MTR_3g098310 | 72 | gi|657395325 | 88029 | 7.91 | 2.90 | 0.030 | |
56 | ATP synthase beta subunit, partial | atpB | 630 | gi|6706178 | 52616 | 4.99 | 11.02 | 0.016 | |
57 | AtpB | atpB | 143 | gi|682124547 | 53618 | 5.13 | 2.12 | 0.025 | |
60 | hexokinase | HXK1 | 115 | gi|110740344 | 53707 | 5.76 | +∞ | 0.034 | |
62 | ATP synthase CF1 alpha subunit | atpA | 253 | gi|313183946 | 55759 | 5.62 | 2.10 | 0.042 | |
Environmental stress response | 3 | C2.6 protein, Universal stress protein family | C2.6 | 64 | gi|33307140 | 18263 | 5.50 | 4.24 | 0.029 |
11 | chaperone protein dnaJ | MTR_2g033460 | 68 | gi|357448821 | 81171 | 9.20 | 6.22 | 0.016 | |
41 | heat shock protein SSB1 | MGG_11513 | 82 | gi|389641395 | 66853 | 5.38 | 3.07 | 0.027 | |
49 | protein enhanced disease resistance 2 | LOC101503113 | 60 | gi|502151333 | 83877 | 6.52 | 7.28 | 0.031 | |
54 | putative disease resistance protein RGA1 | LOC104447499 | 67 | gi|702359424 | 43719 | 5.94 | 6.78 | 0.033 | |
61 | unnamed protein product, Resistant to P. syringae 6 | TRAES_3BF089300150CFD_c1 | 61 | gi|669031836 | 91753 | 8.00 | 3.44 | 0.041 | |
Signal transduction | 10 | hypothetical protein COCSADRAFT_30752, RhoGEF, guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases | COCSADRAFT_30752 | 63 | gi|628085938 | 32647 | 10.25 | 2.42 | 0.039 |
16 | mannan-binding lectin | AF347116 | 81 | gi|13517976 | 28118 | 5.74 | +∞ | 0.030 | |
36 | GDP-mannose-dependent alpha-(1–6)-phosphatidylinositol monomannoside mannosyltransferase | pimB | 93 | gi|664282737 | 40669 | 9.07 | +∞ | 0.024 | |
38 | G-protein coupled receptor 1, partial | KM399174 | 84 | gi|700256424 | 33729 | 9.38 | 3.31 | 0.041 | |
66 | protein synthesis factor GTP-binding protein | HACJB3_RS04355 | 91 | gi|495689754 | 58532 | 5.00 | 5.34 | 0.017 | |
72 | adenylyl cyclase | AT3G21465 | 60 | gi|11994381 | 44065 | 9.07 | 8.61 | 0.019 | |
75 | TonB-linked outer membrane protein, SusC/RagA family | BACCELL_02879 | 66 | gi|224520396 | 126159 | 5.84 | 4.62 | 0.028 | |
76 | spectrin alpha chain-like isoform 2 | LOC100874878 | 80 | gi|383848576 | 280882 | 5.14 | 2.97 | 0.044 | |
77 | GPI mannosyltransferase 2 | F775_16289 | 78 | gi|475571170 | 56255 | 9.30 | 5.14 | 0.034 | |
78 | rho GTPase-activating protein 19 isoform X5 | ARHGAP19 | 80 | gi|114632111 | 52589 | 9.35 | +∞ | 0.025 | |
81 | receptor-like protein kinase 3 | M569_06486 | 106 | gi|527201051 | 68638 | 8.95 | 7.69 | 0.029 | |
82 | GTP binding signal recognition particle protein | ffh | 60 | gi|732152276 | 56257 | 9.11 | +∞ | 0.031 | |
Cytoskeletal | 43 | actin | AF282624 | 203 | gi|9082317 | 41934 | 5.64 | 2.80 | 0.038 |
44 | actin-2 | LOC105059495 | 271 | gi|9082316 | 41646 | 5.23 | 3.58 | 0.025 | |
Amino acid metabolism | 5 | alpha chain of nascent polypeptide associated complex | NbNACa1 | 80 | gi|124484511 | 21911 | 4.32 | 0.26 | 0.026 |
7 | serine racemase | LOC103705524 | 66 | gi|672128011 | 35616 | 6.01 | 3.00 | 0.039 | |
12 | glutamate 5-kinase | MTR_5g042980 | 81 | gi|357487315 | 34715 | 5.86 | 0.47 | 0.031 | |
15 | inner membrane ABC transporter, putative | RCOM_1816900 | 64 | gi|255605404 | 33486 | 9.04 | 0.19 | 0.018 | |
22 | glutamine synthetase precursor | plGS | 101 | gi|5733730 | 48097 | 8.34 | 3.63 | 0.015 | |
28 | ABC transporter C family member 3 | MRP3 | 69 | gi|42572407 | 124952 | 6.40 | 5.45 | 0.029 | |
29 | phenylalanine ammonia lyase | PAL | 71 | gi|58618144 | 77459 | 5.83 | 0.37 | 0.032 | |
39 | Glutamate-ammonia-ligase adenylyltransferase, Bifunctional glutamine-synthetase adenylyltransferase/deadenyltransferase | glnE | 63 | gi|430009452 | 111444 | 5.97 | 0.16 | 0.033 | |
50 | predicted protein, glutaminyl transferase | PHYPADRAFT_184130 | 73 | gi|168024584 | 126285 | 6.81 | 2.46 | 0.037 | |
Sugar transportation | 19 | sugar isomerase domain-containing protein | ARALYDRAFT_485874 | 77 | gi|297322103 | 37783 | 6.51 | 5.45 | 0.031 |
23 | sugar ABC transporter ATP-binding protein | yphE | 74 | gi|501396496 | 28244 | 7.03 | 2.47 | 0.024 | |
31 | monosaccharide ABC transporter ATP-binding protein, CUT2 family | Chro_1853 | 85 | gi|428008801 | 55504 | 5.35 | 3.45 | 0.041 | |
Plant secondary metabolism | 26 | putative oxysterol-binding protein | AWRI1499_2309 | 63 | gi|385303702 | 43378 | 8.78 | 4.03 | 0.046 |
40 | cytochrome P450 | AT4g19230 | 76 | gi|7268718 | 52437 | 8.74 | 2.10 | 0.048 | |
64 | storage protein | AY439332 | 165 | gi|37789212 | 61803 | 5.52 | 0.20 | 0.019 | |
67 | epoxide hydrolase | MTR_7g034950 | 65 | gi|357504133 | 42877 | 5.95 | 3.41 | 0.029 | |
Transcription and translation | 1 | 60S acidic ribosomal protein P3 | POPTR_0009s03780g | 62 | gi|224106463 | 11941 | 4.22 | 6.56 | 0.031 |
9 | ribonuclease | MTR_4g095410 | 63 | gi|357476363 | 41332 | 8.72 | 5.37 | 0.029 | |
51 | helicase, transcription/DNA replication, recombination, and repair | CF70_027305 | 90 | gi|645573106 | 76855 | 5.78 | 0.41 | 0.027 | |
63 | transcription factor 20 | TCF20 | 69 | gi|395540747 | 203758 | 8.84 | 0.38 | 0.019 | |
68 | inhibitor of nuclear factor kappa-B kinase subunit beta | ikbkb | 79 | gi|734610408 | 86822 | 6.15 | 2.19 | 0.012 | |
69 | RNA polymerase sigma factor sigD | LOC18440908 | 67 | gi|586748416 | 50552 | 10.01 | 3.32 | 0.023 | |
70 | hypothetical protein VITISV_028576, chromosome segregation protein SMC | VITISV_028576 | 68 | gi|147818418 | 112276 | 5.01 | 2.25 | 0.031 | |
73 | acetyltransferase, N-Acyltransferase superfamily | EL17_00675 | 82 | gi|660633913 | 25375 | 5.61 | 3.24 | 0.048 | |
Metabolism of cofactors | 65 | formate-tetrahydrofolate ligase | PHEL85_2785 | 63 | gi|697005335 | 60833 | 8.54 | 0.14 | 0.041 |
71 | CoA-transferase | CaiB | 85 | gi|666644607 | 42162 | 5.64 | 2.96 | 0.025 | |
80 | 4-phosphopantetheinyl transferase | DA73_94860 | 60 | gi|692202221 | 23823 | 6.09 | 2.38 | 0.027 | |
Others | 27 | outer membrane assembly lipoprotein YfgL | YfgL | 61 | gi|846382982 | 41113 | 9.13 | 3.15 | 0.036 |
74 | lysophospholipase | Cha6605_1617 | 61 | gi|428016673 | 32143 | 6.84 | 4.10 | 0.028 | |
79 | hypothetical protein, predicted periplasmic protein | — | 69 | gi|575528046 | 53794 | 7.64 | 2.98 | 0.014 | |
83 | membrane protein | — | 77 | gi|739293224 | 27839 | 6.12 | 10.24 | 0.036 |
aSpot numbers refer to the proteins labeled in Fig. 4.
bDiscrepancies exist between the measured and the predicted proteins due to modification or degradation.
cThe values are fold change of proteins in A. lancea induced by AL12 exopolysaccharides compared to control A. lancea plantlets.