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. 2016 Oct 5;6:34735. doi: 10.1038/srep34735

Table 2. Identification of differentially expressed proteins between control A. lancea plantlets and plantlets induced by AL12 exopolysaccharides.

Putative function Spot no.a Protein Gene or gene locus Mascot score Protein ID Mr (Da)b pIb Fold changec p-value
Photosynthesis 4 hypothetical protein PHAVU_007G155500g PHAVU_007G155500g 167 gi|593687543 14047 9.60 2.87 0.031
6 chlorophyll a/b-binding protein CP26 precursor LOC_Os11g13890 152 gi|62733870 24317 5.95 4.35 0.029
14 chlorophyll a/b binding protein precursor AF220527 162 gi|6716783 28542 5.29 2.58 0.047
17 chlorophyll a/b binding protein CAB1 238 gi|5714656 28613 5.29 3.21 0.023
21 chlorophyll a-b binding protein 8 LOC100232927 158 gi|225436257 29511 7.85 2.06 0.036
35 carbonic anhydrase FBU08398 136 gi|40737972 36025 5.85 7.98 0.041
37 ribulose bisphosphate carboxylase large chain rbcL 305 gi|131899 52159 6.13 10.12 0.040
58 ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, partial rbcL 199 gi|1881503 52231 6.03 3.51 0.019
59 ribulose-1,5-bisphosphate carboxylase, partial rbcL 188 gi|2961313 53408 6.05 2.01 0.035
Oxidation-reduction, oxidative burst 2 hypothetical protein GUITHDRAFT_152572, NADH-ubiquinone reductase complex 1 MLRQ subunit GUITHDRAFT_152572 84 gi|551660038 12249 9.95 10.29 0.021
20 putative protein, NADPH-dependent FMN reductase AT4g27270 64 gi|3269288 22355 6.30 4.32 0.048
24 oxygen-evolving enhancer protein 2 LOC103990328 146 gi|695040260 28178 8.61 2.02 0.022
25 photosystem II oxygen-evolving enhancer protein 2 GSCOC_T00013178001 258 gi|661878401 28714 8.22 2.14 0.031
30 N-ethylmaleimide reductase FadH 74 gi|659891198 39341 4.94 2.16 0.037
34 monooxygenase AT4G38540 69 gi|3426064 45505 5.94 2.53 0.020
42 NAD-dependent epimerase/dehydratase MtrDRAFT_AC155890g8v2 81 gi|124360315 31765 7.64 2.23 0.034
45 NADPH: adrenodoxin NGA_0622900 84 gi|553192841 153673 8.64 0.47 0.028
46 gamma-glutamyl putrescine oxidoreductase, putative, partial RCOM_0368500 82 gi|223524108 29290 7.85 2.28 0.029
47 oxygen-evolving enhancer protein 1 PSBO 190 gi|131385 35595 5.84 3.74 0.019
48 hypothetical protein BCCGELA001_06795, partial, COG1032 Fe-S oxidoreductase BCCGELA001_06795 70 gi|404270905 35764 6.13 3.62 0.027
55 NADP-dependent oxidoreductase P1 LOC100282970 88 gi|226528403 38936 6.22 2.29 0.024
Energy metabolism 8 adenylate kinase B ADK-B 72 gi|728811055 27013 6.45 2.44 0.012
13 ATP synthase delta chain LOC104229705 230 gi|698456786 26776 8.94 3.93 0.044
18 butyrate kinase PRK03011 84 gi|499737230 39210 5.81 0.42 0.045
19 protein phosphatase 2A catalytic subunit PP2Ac2 74 gi|350537893 35010 4.78 2.11 0.039
32 phosphoglycerate kinase LOC100249576 111 gi|225464995 50166 8.26 8.20 0.025
33 ATP synthase gamma chain LOC103446721 135 gi|657983988 41501 7.52 3.33 0.031
52 V-type proton ATPase catalytic subunit A AT1G78900 94 gi|332198050 69077 5.29 10.58 0.038
53 ATP-dependent Clp protease MTR_3g098310 72 gi|657395325 88029 7.91 2.90 0.030
56 ATP synthase beta subunit, partial atpB 630 gi|6706178 52616 4.99 11.02 0.016
57 AtpB atpB 143 gi|682124547 53618 5.13 2.12 0.025
60 hexokinase HXK1 115 gi|110740344 53707 5.76 +∞ 0.034
62 ATP synthase CF1 alpha subunit atpA 253 gi|313183946 55759 5.62 2.10 0.042
Environmental stress response 3 C2.6 protein, Universal stress protein family C2.6 64 gi|33307140 18263 5.50 4.24 0.029
11 chaperone protein dnaJ MTR_2g033460 68 gi|357448821 81171 9.20 6.22 0.016
41 heat shock protein SSB1 MGG_11513 82 gi|389641395 66853 5.38 3.07 0.027
49 protein enhanced disease resistance 2 LOC101503113 60 gi|502151333 83877 6.52 7.28 0.031
54 putative disease resistance protein RGA1 LOC104447499 67 gi|702359424 43719 5.94 6.78 0.033
61 unnamed protein product, Resistant to P. syringae 6 TRAES_3BF089300150CFD_c1 61 gi|669031836 91753 8.00 3.44 0.041
Signal transduction 10 hypothetical protein COCSADRAFT_30752, RhoGEF, guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases COCSADRAFT_30752 63 gi|628085938 32647 10.25 2.42 0.039
16 mannan-binding lectin AF347116 81 gi|13517976 28118 5.74 +∞ 0.030
36 GDP-mannose-dependent alpha-(1–6)-phosphatidylinositol monomannoside mannosyltransferase pimB 93 gi|664282737 40669 9.07 +∞ 0.024
38 G-protein coupled receptor 1, partial KM399174 84 gi|700256424 33729 9.38 3.31 0.041
66 protein synthesis factor GTP-binding protein HACJB3_RS04355 91 gi|495689754 58532 5.00 5.34 0.017
72 adenylyl cyclase AT3G21465 60 gi|11994381 44065 9.07 8.61 0.019
75 TonB-linked outer membrane protein, SusC/RagA family BACCELL_02879 66 gi|224520396 126159 5.84 4.62 0.028
76 spectrin alpha chain-like isoform 2 LOC100874878 80 gi|383848576 280882 5.14 2.97 0.044
77 GPI mannosyltransferase 2 F775_16289 78 gi|475571170 56255 9.30 5.14 0.034
78 rho GTPase-activating protein 19 isoform X5 ARHGAP19 80 gi|114632111 52589 9.35 +∞ 0.025
81 receptor-like protein kinase 3 M569_06486 106 gi|527201051 68638 8.95 7.69 0.029
82 GTP binding signal recognition particle protein ffh 60 gi|732152276 56257 9.11 +∞ 0.031
Cytoskeletal 43 actin AF282624 203 gi|9082317 41934 5.64 2.80 0.038
44 actin-2 LOC105059495 271 gi|9082316 41646 5.23 3.58 0.025
Amino acid metabolism 5 alpha chain of nascent polypeptide associated complex NbNACa1 80 gi|124484511 21911 4.32 0.26 0.026
7 serine racemase LOC103705524 66 gi|672128011 35616 6.01 3.00 0.039
12 glutamate 5-kinase MTR_5g042980 81 gi|357487315 34715 5.86 0.47 0.031
15 inner membrane ABC transporter, putative RCOM_1816900 64 gi|255605404 33486 9.04 0.19 0.018
22 glutamine synthetase precursor plGS 101 gi|5733730 48097 8.34 3.63 0.015
28 ABC transporter C family member 3 MRP3 69 gi|42572407 124952 6.40 5.45 0.029
29 phenylalanine ammonia lyase PAL 71 gi|58618144 77459 5.83 0.37 0.032
39 Glutamate-ammonia-ligase adenylyltransferase, Bifunctional glutamine-synthetase adenylyltransferase/deadenyltransferase glnE 63 gi|430009452 111444 5.97 0.16 0.033
50 predicted protein, glutaminyl transferase PHYPADRAFT_184130 73 gi|168024584 126285 6.81 2.46 0.037
Sugar transportation 19 sugar isomerase domain-containing protein ARALYDRAFT_485874 77 gi|297322103 37783 6.51 5.45 0.031
23 sugar ABC transporter ATP-binding protein yphE 74 gi|501396496 28244 7.03 2.47 0.024
31 monosaccharide ABC transporter ATP-binding protein, CUT2 family Chro_1853 85 gi|428008801 55504 5.35 3.45 0.041
Plant secondary metabolism 26 putative oxysterol-binding protein AWRI1499_2309 63 gi|385303702 43378 8.78 4.03 0.046
40 cytochrome P450 AT4g19230 76 gi|7268718 52437 8.74 2.10 0.048
64 storage protein AY439332 165 gi|37789212 61803 5.52 0.20 0.019
67 epoxide hydrolase MTR_7g034950 65 gi|357504133 42877 5.95 3.41 0.029
Transcription and translation 1 60S acidic ribosomal protein P3 POPTR_0009s03780g 62 gi|224106463 11941 4.22 6.56 0.031
9 ribonuclease MTR_4g095410 63 gi|357476363 41332 8.72 5.37 0.029
51 helicase, transcription/DNA replication, recombination, and repair CF70_027305 90 gi|645573106 76855 5.78 0.41 0.027
63 transcription factor 20 TCF20 69 gi|395540747 203758 8.84 0.38 0.019
68 inhibitor of nuclear factor kappa-B kinase subunit beta ikbkb 79 gi|734610408 86822 6.15 2.19 0.012
69 RNA polymerase sigma factor sigD LOC18440908 67 gi|586748416 50552 10.01 3.32 0.023
70 hypothetical protein VITISV_028576, chromosome segregation protein SMC VITISV_028576 68 gi|147818418 112276 5.01 2.25 0.031
73 acetyltransferase, N-Acyltransferase superfamily EL17_00675 82 gi|660633913 25375 5.61 3.24 0.048
Metabolism of cofactors 65 formate-tetrahydrofolate ligase PHEL85_2785 63 gi|697005335 60833 8.54 0.14 0.041
71 CoA-transferase CaiB 85 gi|666644607 42162 5.64 2.96 0.025
80 4-phosphopantetheinyl transferase DA73_94860 60 gi|692202221 23823 6.09 2.38 0.027
Others 27 outer membrane assembly lipoprotein YfgL YfgL 61 gi|846382982 41113 9.13 3.15 0.036
74 lysophospholipase Cha6605_1617 61 gi|428016673 32143 6.84 4.10 0.028
79 hypothetical protein, predicted periplasmic protein 69 gi|575528046 53794 7.64 2.98 0.014
83 membrane protein 77 gi|739293224 27839 6.12 10.24 0.036

aSpot numbers refer to the proteins labeled in Fig. 4.

bDiscrepancies exist between the measured and the predicted proteins due to modification or degradation.

cThe values are fold change of proteins in A. lancea induced by AL12 exopolysaccharides compared to control A. lancea plantlets.