Table 1.
Primers and Probes Used in the Idylla™ Prototype Ebola Virus Test
| Primer Name | Position in Reference Sequence | NCBI/GenBank Accession Number | Oligonucleotide Sequence (5′–3′) |
|---|---|---|---|
| 5EBO-GP_1D (sense) | 6348–6369 | NC_002549 | TGGGCTGAAAAYTGCTACAATC |
| 3EBO-GP_1D (antisense) | 6440–6459 | NC_002549 | CTTTGTGMACATASCGGCAC |
| EBO-GP_1DZ-FIBFQ (antisense) | 6421–6402 | NC_002549 | CCGTCTGGCGCTGCTGGTAG (56-FAM/3IAbkFQ) |
| EBO-GP_1DS-TRIBRQ (antisense) | 6402–6420 | NC_006432 | CATCCGGCGGTGGGGGTAA (5TexRd-XN/3IAbRQSP) |
| PDV FW20 (sense) | 20430–20452 | NC_028249 | GCTGTCTGGGTATACTTCTGATG |
| PDV_Rev20 (antisense) | 20 578–20 599 | NC_028249 | CCTCCCCATTTGTATCTGACTG |
| PDV_Prb20 (antisense) | 20 496–20 519 | NC_028249 | TTGTCATGGTCCCCTTCCTGTGTC (5ATTO647/3IABRQS) |
| RNaseP63fw (sense) | 41–58 | U77665 | CAGATTTGGACCTGCGAG |
| RNaseP63rev (antisense) | 87–103 | U77665 | CGGCTGTCTCCACAAGT |
| RNaseP63Pr (antisense) | 65–83 | U77665 | CTGACCTGAAGGCTCTGCG (5TexRd-XN/3IAbRQSP) |
Abbreviation: NCBI, National Center for Biotechnology Information.