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. 2016 Oct 4;214(Suppl 3):S319–S325. doi: 10.1093/infdis/jiw270

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Published by Oxford University Press for the Infectious Diseases Society of America 2016. This work is written by (a) US Government employee(s) and is in the public domain in the US.

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Figure 2. — Expression of VP40 (A) and glycoprotein (GP; B) in FAM134B^+/+ or FAM134B^−/− cells. MEFs were infected with Ebola virus strain Mayinga (EBOV-Mayinga) or EBOV–Makona GCO7 at a multiplicity of infection (MOI) of 1. Visualization of actin or vinculin (for high-molecular-weight proteins) were used as loading controls. C, Nucleoprotein (NP) staining in EBOV-Makona–infected FAM134B^+/+ or FAM134B^−/− MEFs. Cells were infected with EBOV-Makona at a MOI of 1 for 6 days. Samples were stained for NP (green) and nuclei (DAPI; blue), and images were obtained by confocal microscopy. D, Quantification of infected NP-positive cells.