Table 2.

Limit of Detection (LOD) of Zaire Ebola Virus (ZEBOV) in Whole-Blood Specimens

ZEBOV Level		Replicates, Proportiona	Result
PFU/mL	PFU/test
Preliminary
1 × 107	3 × 105	5/5	Positive
2 × 106	6 × 104	5/5	Positive
1 × 106	3 × 104	5/5	LOD
4 × 105	1.2 × 104	3/5	Negative
1 × 105	3 × 103	0/5	Negative
8 × 104	2.4 × 103	0/5	Negative
1 × 104	300	0/5	Negative
Confirmation
2 × 106	6 × 104	20/20	Positive
1 × 106	3 × 104	20/20	LOD
Positive control	…	2/2	Positive
Negative control	…	0/2	Negative

Abbreviation: PFU, plaque-forming units.

^a Data are no. of positive whole-blood specimens/no. tested.