Table 5.
In Vivo Sampling of Nonhuman Primates Challenged With Zaire Ebola Virus Strain Makona
| Day After Infection, Sample | Clinical Sign(s) | Positive By RDT | Viremia Level, PFU/mL | Real-time qPCR Result, GEq/mL |
|---|---|---|---|---|
| 4 | ||||
| Whole blood | Fever | 2/2 | … | 3.4 × 105 and 5.7 × 106 |
| Plasma | Fever | 2/2 | 6.5 × 104 and 5.23 × 105 | 2.4 × 106 and 1.4 × 107 |
| 6 | ||||
| Whole blood | Fever/hallmark | 2/2 | … | 2 × 108 and 3 × 108 |
| Plasma | Fever/hallmark | 2/2 | 3.05 × 107 and 7.25 × 107 | 6.6 × 108 and 8.8 × 108 |
Cynomolgus macaques were challenged with 1000 PFU/mL Ebola virus strain Makona. Sampling was performed on days 4 and 6 after challenge for RDT and real-time qPCR testing.
Abbreviations: GEq, genome equivalents; PFU, plaque-forming units; qPCR, quantitative polymerase chain reaction; RDT, ReEBOV Antigen Rapid Test.