Figure 1.

Sensitivity of the Ebola virus (EBOV) reverse transcription–loop-mediated isothermal amplification method. Sensitivity was determined against 2 strains of EBOV, EBOV-Kikwit (stock concentration, 1.12 × 10⁷ plaque-forming units [PFU]/mL) and EBOV-Makona (4 × 10⁷ PFU/mL). Ten-fold serial dilutions of each virus were made in human whole blood. Each dilution was added to the lysis buffer at 5%, and 50 µL of this suspension was used to reconstitute lyophilized reagents. Abbreviations: NEG, negative control; POS, positive control (MS2 phage).