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. 2016 Oct 4;214(Suppl 3):S234–S242. doi: 10.1093/infdis/jiw330

Figure 3.

Figure 3.

Performance of the Ebola virus (EBOV) reverse transcription–loop-mediated isothermal amplification method using different levels of sample input. A, Human whole blood was spiked with target (E. colni cells and recombinant EBOV) and added to the lysis buffer at various levels (3.5%–6%). A total of 50 µL of this suspension was used to reconstitute lyophilized reagents, followed by incubation at 72°C for 40 minutes. Means with the same letter are not significantly different from each other (P > .05, by the Tukey-Kramer test,). B, Human whole blood was spiked with target (recombinant EBOV) and added to the lysis buffer at 5%. Various volumes (40–60 µL) of this suspension were used to reconstitute lyophilized reagents, followed by incubation at 72°C for 40 minutes. Means with the same letter are not significantly different from each other (P > .05, by the Tukey-Kramer test).