Table 2.
List of the Pathogens Used for the Evaluation of the Specificity of Ebola Virus (EBOV) Reverse Transcription–Loop-Mediated Isothermal Amplification (RT-LAMP)
| Sample | Isolate | Source | Part Number | Stock Titer | Titer/Test (50 µL) |
|---|---|---|---|---|---|
| 1a | Marburg virus–Angola | UTMB | Not available | 8 × 107 PFU/mL | 2 × 104 PFU/mL |
| 2a | Marburg virus–Musoke | UTMB | Not available | 4.4 × 106 PFU/mL | 1.1 × 103 PFU/mL |
| 3a | Lassa virus | UTMB | Not available | 1.75 × 106 PFU/mL | 4.25 × 102 PFU/mL |
| 4a | Crimean-Congo hemorrhagic fever virus | UTMB | Not available | 1 × 107 PFU/mL | 2.5 × 103 PFU/mL |
| 5 | Respiratory syncytial virus A (2006 isolate) | Zeptometrix | 0810040ACF | 1.95 × 107 U/mL TCID50 | 5.36 × 103 units |
| 6 | Respiratory syncytial virus B (CH93(18)-18) | Zeptometrix | 0810040CF | 1.51 × 106 U/mL TCID50 | 4.16 × 102 units |
| 7 | Influenza A virus (Wisconsin/67/05) | Zeptometrix | 0810252CF | 1.41 × 105 U/mL TCID50 | 3.88 × 101 units |
| 8 | Influenza B virus (Massachusetts/2/12) | Zeptometrix | 0810239CF | 1.41 × 105 U/mL TCID50 | 3.88 × 101 units |
| 9 | Salmonella enterica subspecies arizonae | ATCCd | ATCC 13314 | 1.30 × 108 CFU/vial (1 mL) | 3.58 × 104 CFU |
| 10 | Salmonella enterica serotype Typhi | Zeptometrix | 0801933 | 1.89 × 109 CFU/mL | 5.20 × 105 CFU |
| 11 | Pseudomonas aeruginosa | ATCC | ATCC 10145 | 9.15 × 107 CFU/mL | 2.52 × 104 CFU |
| 12 | Salmonella sonnei | ATCC | ATCC 29930 | >104 CFU/mL | 2.75 × 101 CFU |
| 13 | Plasmodium vivax | ATCC | ATCC 30152 | 4.90 × 106 U/mL TCID50 | 1.35 × 103 units |
| 14 | Plasmodium falciparum | ATCC | ATCC 30932 | 2.84% parasitemia/vial (0.7 mL) | 0.02% parasitemia |
| 15 | Dengue virus serotype 1 (Hawaii) | Zeptometrix | 0810088CF | 2.45 × 105 U/mL TCID50 | 6.74 × 101 units |
| 16 | Dengue virus serotype 2 (New Guinea) | Zeptometrix | 0810089CF | 3.16 × 105 U/mL TCID50 | 8.69 × 101 units |
| 17 | Dengue virus serotype 3 (strain H87) | Zeptometrix | 0810090CF | 1.15 × 107 U/mL TCID50 | 3.16 × 103 units |
| 18 | Dengue virus serotype 4 (H241) | Zeptometrix | 0810091CF | 3.39 × 107 U/mL TCID50 | 9.32 × 103 units |
| 19 | Vibrio cholerae (Z133) | Zeptometrix | 0801902 | 1.90 × 1010 CFU/mL | 5.23 × 106 CFU |
Abbreviations: ATTC, American Type Culture Collection; CFU, colony-forming units; PFU, plaque-forming units; TCID50, 50% tissue culture infective dose; UTMB, University of Texas Medical Branch, Galveston.
a Specificity experiments using these pathogens were done at the UTMB in a biosafety level 4 facility.