Skip to main content
. 2016 Oct 5;6:34504. doi: 10.1038/srep34504

Figure 4. MiR-204 inhibits angiogenesis.

Figure 4

Capillary tubes development was evaluated in vitro using co-cultures of HUVEC and breast cancer cells. (A) MDA-MB-231 and (B) MCF-7 cells plated onto matrigel with serum free medium. (C) HUVEC plated into matrigel with endothelial complete medium. (D) Monoculture of HUVEC in presence of VEGFA (10 ng/ml). (E) Co-culture of HUVEC with MDA-MB-231 or (D) MCF-7 cells transfected with scramble and plated into matrigel with serum free medium for 24 h. (G) Co-culture of HUVEC with MDA-MB-231 or (H) MCF-7 cells transfected with miR-204 (30 nM) and plated into matrigel with serum free medium for 24 h. (I) Mono-cultures of MDA-MB-231 or (J) MCF-7 cells in presence of VEGFA (10 ng/ml). Graphical representation of quantification of branch points (K) and capillary tubes (L) number after 24 h of co-cultures. Data were obtained by two different observers. Results shown are the mean of three independent experiments ± S.D. Arrowheads indicate branch points. Arrows denote capillary-like tubes structures.